Shoot tips can not be regenerated after cryopreservation though there was survival by TTC. That is probably closely related to seedling age、subculture time、growth condition which needed further study.
②茎尖玻璃化超低温保存后经TTC检测有较高存活率,但没有再生,可能与试验材料的苗龄、继代次数,生长状态密切相关,有待进一步研究。
参考来源 - 大花蕙兰‘幻影’组培再生体系建立及离体保存技术研究·2,447,543篇论文数据，部分数据来源于NoteExpress
The thin-skin muskmelon inbred lines were used to study the effects of explants, seedling age, and hormones on plant in vitro regeneration.
以甜瓜薄皮自交系为材料，研究了外植体、苗龄、激素等因素对甜瓜离体再生的影响。
Level of total phenolics of IR36 was increased significantly on seedling age (9d).
诱导IR36，当苗龄为9天时，显著提高了水稻的总酚含量。
Moreover, the seedling age of root-cutting grafted seedling could be shortened to 25 days.
同时，断根嫁接苗苗龄可以缩短到25天。
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